A motion aftereffect induced by a stimulus changing its size produces a perception of motion-in-depth. Similarly, a relative motion of opposite edges presenting subparts of such a stimulus also produces the aftereffect of motion-in-depth. In this study, we examined the spatial and temporal properties of the relative motion for the aftereffect of motion-in-depth. In experiment 1, we used two vertical lines moving away from each other. After adaptation, two stationary test lines were first perceived to be approaching each other (2D-MAE), and subsequently perceived to be moving away from the observer (3D-MAE). This aftereffect gradually decreased as the distance between the two lines increased and then disappeared at about 3 deg/arc apart. In experiment 2, we examined the effect of presenting the two adaptation lines sequentially. The 2D-MAE was induced, but the 3D-MAE could not be found. These results suggest that the aftereffect of motion-in-depth has properties of a spatial and temporal limit.